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In addition to the usual light microscope in the laboratory, which we cannot do without, state-of-the-art confocal microscopy allows us to look at the object in detail by means of a two-stage, magnifying image.

In short, this technique allows us to look at and study membranes on the living cell in extreme detail. This leads to an enormous increase in knowledge and understanding of living processes, which we need in epigenetics and for human therapeutic approaches.

Or to put it in more detail: in this imaging by confocal microscopy, the optics of the microscope have a finite depth of field, similar to the reduced image with a camera. This means that the image of the object is a superposition of a sharp image of the points in the focal plane and an out-of-focus image of points outside the focal plane, but which are still recognised as "sharp" by the detector (eye, camera line). This depth of field prevents the resolution of object details in the axial direction. Confocal imaging reduces this depth of field extremely and allows virtual optical sections through the object with corresponding detail information in the axial direction as well. The trick is point-to-point-to-point imaging.

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Stefan Georgios Moellhausen

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